المرجع الالكتروني للمعلوماتية
المرجع الألكتروني للمعلوماتية
Untitled Document
أبحث عن شيء أخر المرجع الالكتروني للمعلوماتية

مقارنة النماذج الإحصائية مع التجربة
25-12-2021
النشاء
8-6-2017
الفيلم الإلكتروني أو المستشعر Sensor
6-12-2021
Amino Acids Can Act as Acids and Bases
6-4-2017
الفرق بين العولمة والعالمية
19-10-2016
القول اللين
22-04-2015


DNA Polymerases Are the Enzymes That Make DNA


  

2363       11:03 صباحاً       التاريخ: 4-4-2021              المصدر: JOCELYN E. KREBS, ELLIOTT S. GOLDSTEIN and STEPHEN T. KILPATRICK

أقرأ أيضاً
التاريخ: 2-5-2016 4298
التاريخ: 9-7-2018 1336
التاريخ: 9-12-2020 4075
التاريخ: 4-8-2020 2663
التاريخ: 20-5-2019 1530
DNA Polymerases Are the Enzymes That Make DNA

KEY CONCEPTS
- DNA is synthesized in both semiconservative replication and repair reactions.
- A bacterium or eukaryotic cell has several different DNA polymerase enzymes.
- One bacterial DNA polymerase undertakes semiconservative replication; the others are involved in repair reactions.
There are two basic types of DNA synthesis:
- FIGURE 1. shows the result of semiconservative replication. The two strands of the parental duplex are separated, and each serves as a template for synthesis of a new strand. The parental duplex is replaced with two daughter duplexes, each of which has one parental strand and one newly synthesized strand. An enzyme that can synthesize a new DNA strand on a template strand is called a DNA polymerase (or more properly, DNA-dependent DNA polymerase).
- FIGURE 2. shows the consequences of a DNA repair reaction. One strand of DNA has been damaged. It is excised and new material is synthesized to replace it. Both prokaryotic and eukaryotic cells contain multiple DNA polymerase activities.
Only a few of these enzymes actually undertake replication; those that do sometimes are called DNA replicases. The remaining enzymes are involved in repair synthesis  or participate in subsidiary roles in replication.
src=../../../medea/images/2x_599.jpg
FIGURE 1. Semiconservative replication synthesizes two new strands of DNA.
src=../../../medea/images/3_537.jpg
FIGURE 2.  Repair synthesis replaces a short stretch of one strand of DNA containing a damaged base.
All prokaryotic and eukaryotic DNA polymerases share the same fundamental type of synthetic activity, antiparallel synthesis from 5′ to 3′ from a template that is 3′ to 5′. This means adding nucleotides one at a time to a 3′–OH end, as illustrated in FIGURE 3. The choice of the nucleotide to add to the chain is dictated by base pairing with the complementary template strand.
src=../../../medea/images/2x_600.jpg
FIGURE 3. DNA is synthesized by adding nucleotides to the 3′–OH end of the growing chain, so that the new chain grows in the 5′ to 3′ direction. The precursor for DNA synthesis is a nucleoside triphosphate, which loses the terminal two phosphate groups in the reaction.
Some DNA polymerases, such as the repair polymerases, function as independent enzymes, whereas others (notably the replication polymerases) are incorporated into large protein assemblies called holoenzymes. The DNA-synthesizing subunit is only one of several functions of the holoenzyme, which typically contains other activities concerned with fidelity.
TABLE 1 summarizes the DNA polymerases that have been characterized in E. coli. DNA polymerase III, a multisubunit protein, is the replication polymerase responsible for de novo synthesis of new strands of DNA. DNA polymerase I (encoded by polA) is involved in the repair of damaged DNA and, in a subsidiary role, in semiconservative replication. DNA polymerase II is required to restart a replication fork when its progress is blocked by damage in
DNA. DNA polymerases IV and V are involved in allowing replication to bypass certain types of damage and are called errorprone polymerases.
TABLE 11.1 Only one DNA polymerase is the replication enzyme. The others participate in repairing damaged DNA, restarting stalled replication forks, or bypassing damage in DNA.
src=../../../medea/images/2x_601.jpg
When researchers assay extracts of E. coli for their ability to synthesize DNA, the predominant enzyme activity is DNA polymerase I. Its activity is so great that it makes it impossible to  detect the activities of the enzymes actually responsible for DNA replication! To develop in vitro systems in which replication can be followed, researchers therefore prepare extracts from polA mutant cells.
Several classes of eukaryotic DNA polymerases have been identified. DNA polymerases δ and ε are required for nuclear replication; DNA polymerase α is concerned with “priming” (initiating) replication. Other DNA polymerases are involved in repairing damaged nuclear DNA, or in translesion replication of damaged DNA when repair of damage is impossible. Mitochondrial DNA replication is carried out by DNA polymerase γ, whereas chloroplasts have their own replication system.


Untitled Document
جواد مرتضى
عراقيّةٌ أنجبَتْهَا الفحولةُ من العربِ
د. فاضل حسن شريف
اشارات قرآنية من كتاب باب الحوائج الإمام الكاظم...
عبدالله مرتضى محمد تقي الحسيني
المبعث النبوي: نور ورحمة وهداية
د. فاضل حسن شريف
اشارات قرآنية من كتاب باب الحوائج الإمام الكاظم...
ياسين فؤاد الشريفي
الموز الأزرق!
جواد مرتضى
حرمة الخمر قرآنيا
جواد مرتضى
السيّد مُحمّد سبعُ الدُّجَيل
حسن علاء
لقيط أبو رزين المنتفقي
د. فاضل حسن شريف
اشارات الامام موسى الكاظم عليه السلام عن القرآن...
جواد مرتضى
نصرة الامام الكاظم لأهل البيت (عليهم السلام)
د. فاضل حسن شريف
اشارات الامام موسى الكاظم عليه السلام عن القرآن...
الشيخ أحمد الساعدي
مصابك يا موسى أبن جعفر (ع) أدمى قلوبنا
د. فاضل حسن شريف
اشارات الامام الهادي عليه السلام عن القرآن الكريم من...
جواد مرتضى
السفير الثاني: محمد بن عثمان العمريّ الخلّانيّ