Peptides Can Be Distinguished by Their Ionization Behavior

Peptides contain only one free α-amino group and one free α-carboxyl group, at opposite ends of the chain (Fig. 1.1). These groups ionize as they do in free amino acids, although the ionization constants are different because an oppositely charged group is no longer linked to the α carbon. The α-amino and α-carboxyl groups of all nonterminal amino acids are covalently joined in the peptide bonds, which do not ionize and thus do not contribute to the total acid-base behavior of peptides.

FIGURE 1.1 Alanylglutamylglycyllysine. This tetrapeptide has one free α-amino group, one free α-carboxyl group, and two ionizable R groups. The groups ionized at pH 7.0 are in red.

However, the R groups of some amino acids can ionize and in a peptide these contribute to the overall acid-base properties of the molecule (Fig. 1.1). Thus the acid-base behavior of a peptide can be predicted from its free α-amino and α-carboxyl groups as well as the nature and number of its ionizable R groups. Like free amino acids, peptides have characteristic titration curves and a characteristic isoelectric pH (pI) at which they do not move in an electric field. These properties are exploited in some of the techniques used to separate peptides and proteins, as we shall see later in the chapter. It should be emphasized that the pKa value for an ionizable R group can change somewhat when an amino acid becomes a residue in a peptide. The loss of charge in the α-carboxyl and α-amino groups, the interactions with other peptide R groups, and other environmental factors can affect the pKa. s.

مواضيع ذات صلة

تصنيف البروتينات classification of proteins

الدنترة (المسخ) Denaturation والتغيرات التي تطرأ على البروتين الممسوخ

التنظيمات البنائية التركيبية للبروتين

البروتينات proteints

الببتيدات Peptides

الأحماض الامينية غير البروتنيية

تصنيف الأحماض الامينية Classification of amino acids

الخصائص الحامضية - القاعدية للأحماض الامينية The acid-base properties of amino acids

الفعالية البصرية للأحماض الامينية Optical activity of amino acids

الأحماض الامينية

محلل الأحماض الأمينية التلقائي Automated amino acid analyzer

تقدير الوزن الجزيئي للبروتينات