Molecular Fluorescence Instrumental : Part 1: Approximate Excitation Wavelength

The instrument you'll be using in this portion of the experiment is an Agilent HP8453 UV-Vis spectrometer.

The first step in conducting the analysis is to determine the optimum excitation wavelength for riboflavin. When no prior information is available, as in this case, the absorption spectrum can be used to approximate the best excitation wavelength.

Look near the Agilent HP8453 UV-Vis spectrometer for a pack of cuvettes. If you cannot find a pack, ask your T.A. for a new pack. Use the 10 ppm solution to obtain an absorption spectrum of riboflavin from 280 to 600 nm. Use distilled water as your reference solution. If you do not recall how to acquire an absorbance spectrum, check with your TA for instructions. The wavelength of maximum absorbance should be used as an initial excitation wavelength. There may be more than one absorbance peak.

*Print the absorbance spectrum*