HPLC as a solution to efficiency problems

While all of these basic principles hold true for all chromatographic separations, HPLC was developed as method to solve some of the shortcomings of standard liquid chromatography. Classic liquid chromatography has several severe limitations as a separation method. When the solvent is driven by gravity, the separation is very slow, and if the solvent is driven by vacuum, in a standard packed column, the plate height increases and the effect of the vacuum is negated. The limiting factor in liquid chromatography was originally the size of the column packing, once columns could be packed with particles as small as 3 µm, faster separations could be performed in smaller, narrower, columns. High pressure was required to force the mobile phase and sample through these new columns, and previously unneeded apparatus was required to maintain reproducibility of results in this new instruments. The use of high pressures in a narrow column allowed for a more effective separation to be achieved in much less time than was required for previous forms of liquid chromatography.

مواضيع ذات صلة

التحليل الطيفي

الإشعاع الكهرومغناطيسي Electromagnetic Radiation

مكونات الجهاز المستخدم في التحليل الالي

طرق التحليل الآلي Instrumental Methods) )

اطياف الاشعة تحت الحمراء

تشتت الدوران البصري والانكسار المزدوج الدائري

مطياف الفلورة (التألق) Fluorescence spectroscopy

الضوء المستقطب والنشاط الضوئي

الدوران النوعي

البولاريمتر

Electrophoretic Mobility 2

Capillary Electrophoresis 2