DNA Polymerase Holoenzyme Consists of Subcomplexes

KEY CONCEPTS
- The E. coli DNA polymerase III catalytic core contains three subunits, including a catalytic subunit and a proofreading subunit.
- The DNA Pol III holoenzyme has at least two catalytic cores, a processivity clamp, and a dimerization clamploader complex.
- A clamp loader places the processivity subunits on DNA,where they form a circular clamp around the nucleic acid.
- At least one catalytic core is associated with each template strand.
- The E. coli replisome is composed of the holoenzyme complex and the additional enzymes required for chromosome replication.
We can now relate the subunit structure of E. coli DNA polymerase III holoenzyme (also called a replisome) to the activities required for DNA synthesis and propose a model for its action. The replisome consists of the DNA polymerase III holoenzyme complex and associated proteins, primase and helicase, necessary for replication function. A new model for the structure of the DNA Pol III complex proposes a three-polymerase core structure, with two Pol III catalytic cores responsible for synthesis of the lagging strand and one for the leading strand. Each Okazaki fragment is synthesized by a new alternating core polymerase. The holoenzyme is a complex of 900 kD that contains 10 different proteins organized into four types of subcomplex:
- There are at least two copies of the catalytic core. Each catalytic core contains the α subunit (the DNA polymerase activity), the ε subunit (the 3′–5′ proofreading exonuclease), and the θ subunit (which stimulates the exonuclease).
- There are two copies of the dimerizing subunit, τ, which link the two catalytic cores together.
There are two copies of the clamp, which is responsible for holding catalytic cores onto their template strands. Each clamp consists of a homodimer of β subunits, the β ring, which binds around the DNA and ensures processivity.
- The γ complex is a group of seven proteins, encoded by five genes that comprise the clamp loader; the clamp loader places the β clamp on DNA by opening the ring.
FIGURE 1 shows one of the models for the assembly of DNA polymerase III. The holoenzyme assembles on DNA in three stages:
- First, the clamp loader uses hydrolysis of ATP to bind β subunits to a template-primer complex.
- Binding to DNA changes the conformation of the site on β that binds to the clamp loader, and as a result it now has a high affinity for the core polymerase. This enables core polymerase to bind, and this is the means by which the core polymerase is brought to DNA.
- A τ dimer binds to the core polymerase and provides a dimerization function that binds a second core polymerase (associated with another β₂ clamp). The replisome is an asymmetric dimer because it has only one clamp loader and (at least) two copies of the catalytic core. The clamp loader is responsible for adding a pair of β₂ dimers to each parental strand of DNA.

FIGURE 1. DNA polymerase III holoenzyme assembles in stages, generating an enzyme complex that synthesizes the DNA of both new strands.
Each of the core complexes of the holoenzyme synthesizes one of the new strands of DNA. The clamp loader is also needed for unloading the β clamp from DNA; as a result, the two cores have different abilities to dissociate from DNA. This corresponds to the need to synthesize a continuous leading strand (where polymerase remains associated with the template) and a discontinuous lagging strand (where polymerase repetitively dissociates and reassociates). The clamp loader is associated with the core polymerase that synthesizes the lagging strand and plays a key role in the ability to synthesize individual Okazaki fragments.

مواضيع ذات صلة

Heterochromatin Formation Requires MicroRNAs

How Does RNA Interference Work

MicroRNAs Are Widespread Regulators in Eukaryotes

Bacteria Contain Regulator RNAs

Introduction to Regulatory RNA

Noncoding RNAs Can Be Used to Regulate Gene Expression

A Riboswitch Can Alter Its Structure According to Its Environment

Introduction to Noncoding RNA

Prions Cause Diseases in Mammals

Oppositely Imprinted Genes Can Be Controlled by a Single Center

DNA Methylation Is Responsible for Imprinting

Chromosome Condensation Is Caused by Condensins