The Biological Reaction of Biosensor

An important factor in most biosensor configurations is the sensing surface. This normally consists of a thin layer of biologically active material in intimate contact with the electronic or optical transducer. In some cases the biological material may be covalently or non-covalently attached to the surface, but often in electrochemical biosensors it forms part of a thin membrane covering the sensing surface. Generally, the conversion of the biological process into an electronic or optical signal is most efficient where there is minimal distance between where the biological reaction or binding takes place and where the electronic transduction takes place.

In addition, it is important for the retention of biological activity that the biological material is not lost into analyte solutions. The immobilisation technology for holding the biocatalyst in place is extensive, with examples of immobilisation methods summarised in Table . Much current research is directed at stabilising enzymes for use in biosensors. At a suitable pH, polyelectrolytes such as diethylaminoethyldextran wrap around enzymes, restricting their movement and reducing their tendency to denature. Polyelectrolytes in combination with polyalcohols, such as sorbitol and lactitol, have been shown to stabilise enzymes considerably against thermal inactivation for use in biosensors. More recently, enzymes have been specifically engineered for their use in biosensors.

Table . Examples of biosensor immobilisation methods.

مواضيع ذات صلة

The Biology of Restriction Enzymes

The Isolation of DNA

MODERN BIOTECHNOLOGY

ANCIENT BIOTECHNOLOGY

DEFINITIONS OF BIOTECHNOLOGY

Future Perspectives of Biofuels and Biotechnology

Biobutanol

Ethanol Production from Biomass

Improved Production of Corn Ethanol

Application of Biotechnology Tools to Biofuels Processes

Biodiesel

Production of The Major Biofuels