Aqueous Two-phase Extraction

The process of aqueous two-phase extraction has recently attracted the attention of investigators wishing to develop alternatives to traditional packed bed chromatographic procedures. This has been particularly evident in the purification of plant-manufactured biopharmaceuticals.

Aqueous two-phase systems integrate the steps of cell disruption with product recovery using either polymer-based systems, hydrophobic and affinity precipitation or the use of surfactants. The theory is that the protein of interest and cellular debris and other material will exhibit differential solubility between two aqueous phases. The two phases are usually created by mixing solutions of polyethylene glycol with either dextran or salts to form two immiscible layers or phases. Despite the fact that aqueous two-phase extraction can be applied to very crude mixtures while providing high capacity and easy scale-up, its use to date has been limited to selected industrial applications. This is largely due to the high cost of the polymers used and a lack of fully developed design approaches. Novel polymers are currently being developed that may overcome such limitations, especially the development of low-cost starch- and cellulose-based polymers for some of the more attractive uses of aqueous two-phase systems such as affinity precipitation.

مواضيع ذات صلة

The Biology of Restriction Enzymes

The Isolation of DNA

MODERN BIOTECHNOLOGY

ANCIENT BIOTECHNOLOGY

DEFINITIONS OF BIOTECHNOLOGY

Future Perspectives of Biofuels and Biotechnology

Biobutanol

Ethanol Production from Biomass

Improved Production of Corn Ethanol

Application of Biotechnology Tools to Biofuels Processes

Biodiesel

Production of The Major Biofuels